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KMID : 0043319980210020135
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Archives of Pharmacal Research
1998 Volume.21 No. 2 p.135 ~ p.139
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Production of Antibody against Saikosaponin a, an Active Component of Bupleuri Radix
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Jung Da-Woon
Sung Chung-Ki
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Abstract
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High titer rabbit polyclonal antibodies (pAbs) which show a specificity for saikosaponin a (SSA), have been generated. The immunogen used was a conjugate of SSA linked through its glucose moiety to bovine serum albumin by periodate oxidation method. The antibody titers obtained from two rabbits, innoculated with the immunogen, reached a plateau after the fourth and third booster injection, respectively. The specificity of the pAbs was determined by hapten inhibition assays using several SSA-like structures. SSA competitively inhibited the binding of the rabbit anti-SSA pAbs to SSA-ovalbumin on solid phase, a coated antigen on the well. The antibodies showed high specificity to SSA, exhibiting no significant cross-reactivity with any of SSA analogues tested.
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KEYWORD
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Saikosaponin a, Polyclonal antibodies, Periodate oxidation, Hapten inhibition assay
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